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Cell surface markers on canine lymphocytes.

C H Miller, A R Carbonell, R Peng

    American Journal of Veterinary Research
    |July 1, 1978
    PubMed
    Summary
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    Canine T lymphocytes and B lymphocytes were quantified in various tissues using rosette assays. The E rosette technique effectively identifies canine T lymphocytes and monitors their populations.

    Area of Science:

    • Immunology
    • Veterinary Science

    Background:

    • Accurate enumeration of lymphocyte populations is crucial for understanding canine immune function.
    • T lymphocytes and B lymphocytes play distinct roles in cell-mediated and humoral immunity, respectively.

    Purpose of the Study:

    • To establish reference values for T and B lymphocytes in canine lymphoid tissues.
    • To evaluate the utility of erythrocyte (E) rosette and erythrocyte-antibody-complement (EAC) rosette assays for canine lymphocyte identification.
    • To assess the impact of mitogen stimulation on lymphocyte populations.

    Main Methods:

    • Lymphocytes were isolated from canine thymus, spleen, lymph node, bone marrow, and peripheral blood.
    • E rosette assay with human erythrocytes was used to identify T lymphocytes.
    • EAC rosette assay and direct fluorescent technique for surface immunoglobulins were used to identify B lymphocytes.

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  • Peripheral blood lymphocytes were stimulated with phytohemagglutinin (PHA) and concanavalin A (ConA).

    • Main Results:

    • T lymphocyte percentages ranged from 1% (thymus) to 13% (peripheral blood) via E rosette assay.
    • B lymphocyte percentages ranged from 3% to 41% (EAC rosette) and 6% to 36% (surface immunoglobulins) across tissues.
    • Mitogen stimulation (PHA, ConA) increased E-rosetting cells (T lymphocytes) and decreased EAC-rosetting cells (B lymphocytes).
    • PHA stimulation led to an increased percentage of Fc receptor-bearing cells.

    Conclusions:

    • The E rosette technique is a reliable method for identifying and monitoring canine T lymphocytes.
    • Lymphocyte distribution varies significantly across different canine lymphoid tissues.
    • Mitogen stimulation differentially affects T and B lymphocyte populations, providing insights into immune responses.