Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Clinical applications of flow cytometry.

M N Raber1

  • 1Division of Medicine, MD Anderson Hospital and Tumor Institute.

Oncology (Williston Park, N.Y.)
|March 1, 1988
PubMed
Summary

Flow cytometry analyzes single cells to reveal tumor heterogeneity, aiding in prognosis and classification. This technique is vital for understanding cell ploidy, lymphoid differentiation, and detecting minimal residual disease.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Carcinoma of unknown primary with gastrointestinal profile: immunohistochemistry and survival data for this favorable subset.

International journal of clinical oncology·2013
Same author

A patient's perspective on cancer-related fatigue.

Cancer·2001
Same author

Classification and regression tree analysis of 1000 consecutive patients with unknown primary carcinoma.

Clinical cancer research : an official journal of the American Association for Cancer Research·1999
Same author

Clinical results of a pharmacodynamically-based strategy for higher dosing of gemcitabine in patients with solid tumors.

Annals of oncology : official journal of the European Society for Medical Oncology·1998
Same author

Unknown primary tumors metastatic to liver.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·1998
Same author

Detection of primary breast cancer presenting as metastatic carcinoma of unknown primary origin by 111In-pentetreotide scan.

Annals of oncology : official journal of the European Society for Medical Oncology·1998

Area of Science:

  • Biomedical science
  • Cell biology
  • Oncology

Background:

  • Tumors exhibit clinical heterogeneity, mirroring biologic variations at the cellular level.
  • Flow cytometry offers multi-parametric analysis of individual cells, crucial for dissecting this heterogeneity.

Purpose of the Study:

  • To highlight the utility of flow cytometry in characterizing cellular heterogeneity in tumors.
  • To underscore its role in prognostic assessment and disease classification.

Main Methods:

  • Single-cell analysis using flow cytometry.
  • Assessment of DNA content for cell ploidy.
  • Analysis of various cellular markers.

Main Results:

  • Flow cytometry elucidates cellular heterogeneity, providing a biologic basis for clinical tumor variations.
  • DNA content analysis aids in establishing prognosis for solid tumors.
  • Marker analysis advances understanding of lymphoid differentiation and hematologic malignancies.

Conclusions:

  • Flow cytometry is a powerful tool for characterizing tumor heterogeneity.
  • It plays a critical role in prognosis, classification, and detection of minimal residual disease in hematologic malignancies and solid tumors.

Related Experiment Videos