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Published on: October 20, 2016
Comprehensive data analysis for development of custom qRT-PCR miRNA assay for glioblastoma: a prevalidation study
Eman A Toraih1,2, Hoda Y Abdallah1,2, Essam A Rashed3,4
1Department of Histology & Cell Biology, Genetics Unit, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt.
Aim:
Glioblastoma (GB) is one notable example of miRNA-modulated neoplasms. Given its unique expression signature, proper miRNA profiling can help discriminate between GB and other types of brain tumors. The current work aimed to develop a more GB-specific and applicable custom designed quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) miRNA assay.
Materials & Methods:
A comprehensive data analysis of bioinformatics databases, previous literature and commercially available pre-designed miRNA PCR arrays within the market.
Results:
A highly enriched panel of 84 deregulated and GB-specific miRNAs has been developed.
Conclusion:
After validation of this newly developed array, it can not only save the researcher's time and effort, but can also have a potential diagnostic and/or prognostic role in GB, paving the road toward personalized medicine.
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