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Related Experiment Video

Updated: Jan 28, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
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Modified Vogel and Johnson Agar for Staphylococcus aureus.

Gail P Andrews1, Scott E Martin1

  • 1Department of Food Science, University of Illinois, Urbana, Illinois 61801.

Journal of Food Protection
|February 24, 2019
PubMed
Summary
This summary is machine-generated.

A modified Vogel and Johnson Agar, enhanced with beef extract, deoxyribonucleic acid, phosphatidyl choline, and catalase, effectively enumerates stressed Staphylococcus aureus. This new medium matches Baird-Parker Agar

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Area of Science:

  • Microbiology
  • Food Science
  • Clinical Diagnostics

Background:

  • Vogel and Johnson Agar is a standard medium for microbial enumeration.
  • Accurate enumeration of Staphylococcus aureus is critical for food safety.
  • Staphylococcus aureus can become stressed under certain conditions, potentially affecting its detectability.

Purpose of the Study:

  • To present and evaluate a modified Vogel and Johnson Agar formulation.
  • To assess the efficacy of the modified medium for enumerating stressed Staphylococcus aureus.
  • To compare the performance of the modified medium with Baird-Parker Agar.

Main Methods:

  • A modified Vogel and Johnson Agar was formulated by adding beef extract, deoxyribonucleic acid, phosphatidyl choline, and catalase.
  • The modified medium was used for the enumeration of stressed Staphylococcus aureus.
  • Enumeration results were compared to those obtained using Baird-Parker Agar.

Main Results:

  • The modified Vogel and Johnson Agar formulation was successfully developed.
  • The new medium demonstrated effectiveness comparable to Baird-Parker Agar.
  • The modified medium accurately enumerated stressed Staphylococcus aureus from processed food samples.

Conclusions:

  • The modified Vogel and Johnson Agar is a viable alternative to Baird-Parker Agar.
  • This enhanced medium supports the reliable enumeration of stressed Staphylococcus aureus in food matrices.
  • The findings contribute to improved methods for microbiological analysis in food safety.