Related Experiment Video
Updated: Jan 28, 2026
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
Published on: October 18, 2010
13.5K
Effectiveness of Sampling Methods for Salmonella Detection on Processed Broilers
N A Cox1, A J Mercuri1, D A Tanner1
1Animal Products Laboratory, Richard B. Russell Agricultural Research Center, USDA, ARS, Athens, Georgia 30604.
Journal of Food Protection
|February 24, 2019
Summary
Whole-carcass sampling detected Salmonella in 45% of broiler carcasses, significantly more than neck-skin methods (11-12%). Pre-enrichment did not improve Salmonella detection rates.
Area of Science:
- Food Safety
- Microbiology
- Poultry Science
Background:
- Salmonella contamination in broiler carcasses is a significant food safety concern.
- Accurate detection methods are crucial for controlling Salmonella spread in poultry processing.
Purpose of the Study:
- To compare the efficacy of different Salmonella detection methods in processed broiler carcasses.
- To evaluate whole-carcass versus neck-skin sampling techniques.
- To assess the impact of destructive versus non-destructive sampling and pre-enrichment on Salmonella detection.
Main Methods:
- 240 processed broiler carcasses were sampled using whole-carcass rinse, neck-skin rinse, and macerated neck skin.
- Destructive and non-destructive sampling methods were compared.
- The effect of pre-enrichment versus direct enrichment was evaluated for Salmonella detection.
Main Results:
- Whole-carcass sampling identified Salmonella in 45% of carcasses, significantly higher than neck-skin methods (11-12%).
- No significant difference was found between destructive and non-destructive whole-carcass sampling.
- Rinsing and blending neck-skin samples yielded comparable results.
- Pre-enrichment did not significantly increase the detection rate of Salmonella.
Conclusions:
- Whole-carcass sampling is significantly more effective for detecting Salmonella in broiler carcasses than neck-skin methods.
- Sampling and enrichment strategies evaluated did not significantly impact Salmonella detection rates, except for the choice of carcass area sampled.
Related Concept Videos
01:18Effects of EDTA on End-Point Detection Methods
660
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
660
01:18Sampling Methods: Sample Types
3.2K
Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
3.2K
00:55Convenience Sampling Method
11.4K
Sampling is a technique to select a portion (or subset) of the larger population and study that portion (the sample) to gain information about the population. Data are the result of sampling from a population. The sampling method ensures that samples are drawn without bias and accurately represent the population.
Convenience sampling is a non-random method of sample selection; this method selects individuals that are easily accessible and may result in biased data. For example, a marketing...
Convenience sampling is a non-random method of sample selection; this method selects individuals that are easily accessible and may result in biased data. For example, a marketing...
11.4K
01:18The Scientific Method in Nursing Process
17.5K
The scientific method provides the foundation for any research. It is the most reliable and objective of all forms of gaining knowledge and guides in applying research-based evidence in practice and conducting future research.
When using research findings to change practice, one must understand the process used to guide a study. The scientific method is a systematic, step-by-step process that supports the data's validity, reliability, and generalizability. As a result, findings can be...
When using research findings to change practice, one must understand the process used to guide a study. The scientific method is a systematic, step-by-step process that supports the data's validity, reliability, and generalizability. As a result, findings can be...
17.5K
01:06Sampling Methods: Overview
3.4K
A sample refers to a smaller subset representative of a larger population. In analytical chemistry, studying or analyzing an entire population is often impractical or impossible. Therefore, samples are used to draw inferences and generalize the whole population. The sampling method selects individuals or items from a population to create a sample. Standard sampling methods include random, judgemental, systematic, stratified, and cluster sampling.
In analytical chemistry, the choice of...
In analytical chemistry, the choice of...
3.4K
01:17Systematic Sampling Method
13.0K
Sampling is a technique to select a portion (or subset) of the larger population and study that portion (the sample) to gain information about the population. Data are the result of sampling from a population. The sampling method ensures that samples are drawn without bias and accurately represent the population. Because measuring the entire population in a study is not practical, researchers use samples to represent the population of interest.
Systematic sampling is one of the simplest methods...
Systematic sampling is one of the simplest methods...
13.0K