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Updated: Jan 28, 2026

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation
Published on: October 31, 2019
Draft genome sequence of Enterococcus faecium SP15, a potential probiotic strain isolated from spring water
Fauzia Aziz1,2,3, Muhammad Nasim Khan4, Safia Ahmed5
1Department of Zoology, The University of Azad Jammu and Kashmir, Muzaffarabad, Azad Kashmir, Pakistan. f.aziz@reading.ac.uk.
This study sequenced the genome of Enterococcus faecium SP15, a novel probiotic strain from spring water. The analysis revealed its potential for producing antimicrobial enterocins without harmful virulence factors.
Area of Science:
- Microbiology
- Genomics
Background:
- Enterococci are Gram-positive bacteria found in the gastrointestinal tract and various environments.
- Enterococcus faecium can produce antimicrobial compounds called enterocins, exhibiting probiotic potential.
Purpose of the Study:
- To analyze the genome of a newly identified enterocin-producing strain, Enterococcus faecium SP15.
- To understand the antimicrobial and probiotic properties of E. faecium SP15 through genomic insights.
Main Methods:
- Genome sequencing of E. faecium SP15.
- Bioinformatic analysis to identify genetic loci for enterocin production and detect virulence factors and antibiotic resistance genes.
Main Results:
- The draft genome of E. faecium SP15 has a size of 2,783,033 bp with a 38.08% G+C content.
- Five genetic loci associated with enterocin production were identified.
- No virulence factors were detected, and only two potential antibiotic resistance genes were found.
Conclusions:
- The genome analysis supports the potential of E. faecium SP15 as a probiotic agent.
- The absence of virulence factors and limited antibiotic resistance genes suggest a favorable safety profile for E. faecium SP15.
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