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Related Experiment Video

Updated: Jan 28, 2026

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
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Ssu72 phosphatase is a conserved telomere replication terminator.

Jose Miguel Escandell1, Edison Sm Carvalho2, Maria Gallo-Fernandez2

  • 1Instituto Gulbenkian de Ciência, Oeiras, Portugal jplanells@igc.gulbenkian.pt Miguel-Godinho.FERREIRA@unice.fr.

The EMBO Journal
|February 24, 2019
PubMed
Summary

The conserved phosphatase Ssu72 terminates telomere replication by regulating Stn1 phosphorylation and recruitment. This ensures proper lagging-strand DNA synthesis, maintaining telomere length homeostasis in yeast and human cells.

Keywords:
CSTSSU72fission yeastlagging‐strand synthesistelomere

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Telomeres protect chromosome ends but their replication is complex.
  • Telomere replication involves DNA polymerases and telomerase.
  • Regulation of telomere replication, especially termination, is not fully understood.

Purpose of the Study:

  • To investigate the role of the conserved phosphatase Ssu72 in telomere replication.
  • To elucidate the mechanism by which Ssu72 regulates telomere replication termination.

Main Methods:

  • Investigated Ssu72 function in fission yeast telomere replication.
  • Analyzed Stn1 phosphorylation and recruitment to telomeres.
  • Examined telomere replication defects in ssu72∆ mutants.
  • Assessed hSSU72 and hSTN1 roles in human cells.

Main Results:

  • Ssu72 terminates telomere replication by regulating Stn1 phosphorylation at Ser74.
  • Ssu72 controls Stn1 recruitment to telomeres.
  • ssu72∆ mutants show defective telomere replication and long 3'-ssDNA overhangs.
  • hSSU72 regulates telomerase activation by controlling hSTN1 recruitment in human cells.

Conclusions:

  • Ssu72 is a conserved phosphatase critical for terminating telomere replication.
  • Ssu72 ensures proper lagging-strand DNA synthesis and telomere homeostasis.
  • This study reveals a novel role for SSU72 in regulating telomere replication and telomerase activation.