Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glucuronidation and sulfation in rabbit kidney.

J T Hjelle, G A Hazelton, C D Klaassen

    The Journal of Pharmacology and Experimental Therapeutics
    |January 1, 1986
    PubMed
    Summary

    The study found that UDP-glucuronosyltransferase and sulfotransferase activities are concentrated in mammalian kidney proximal tubules. These enzymes are crucial for drug metabolism and detoxification within the kidney.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Degradation of Keap1 activates BH3-only proteins Bim and PUMA during hepatocyte lipoapoptosis.

    Cell death and differentiation·2014
    Same author

    Polychlorinated biphenyl congeners that increase the glucuronidation and biliary excretion of thyroxine are distinct from the congeners that enhance the serum disappearance of thyroxine.

    Drug metabolism and disposition: the biological fate of chemicals·2011
    Same author

    Application of multivariate statistical procedures to identify transcription factors that correlate with MRP2, 3, and 4 mRNA in adult human livers.

    Xenobiotica; the fate of foreign compounds in biological systems·2009
    Same author

    Induction of rat UDP-glucuronosyltransferases in liver and duodenum by microsomal enzyme inducers that activate various transcriptional pathways.

    Drug metabolism and disposition: the biological fate of chemicals·2006
    Same author

    Alterations in transporter expression in liver, kidney, and duodenum after targeted disruption of the transcription factor HNF1alpha.

    Biochemical pharmacology·2006
    Same author

    Induction of genes for metabolism and transport by trans-stilbene oxide in livers of Sprague-Dawley and Wistar-Kyoto rats.

    Drug metabolism and disposition: the biological fate of chemicals·2006

    Area of Science:

    • Renal Physiology
    • Drug Metabolism
    • Enzymology

    Background:

    • Mammalian kidneys possess diverse cell types with varied functions.
    • Understanding enzyme distribution is key to renal physiology and xenobiotic metabolism.

    Purpose of the Study:

    • To determine the intrarenal distribution of UDP-glucuronosyltransferase (UGT) and sulfotransferase (SULT) activities.
    • To investigate the localization of these enzymes within kidney tissue.

    Main Methods:

    • Dissection of female rabbit kidneys into cortex, outer medulla, and proximal tubules.
    • Isolation of proximal tubule segments (S2 region) via mechanical methods.
    • Assay of UGT and SULT activities using 1-naphthol as a substrate.

    Main Results:

    • UGT and SULT activities were highest in proximal tubules compared to outer stripe and cortex.
    • Detergents affected glucuronidation differently across kidney regions.
    • Subcellular fractionation indicated UGT and SULT are localized in endoplasmic reticulum/Golgi and cytosol, respectively.
    • UDP-glucose dehydrogenase activity was highest in proximal tubules.

    Conclusions:

    • Proximal tubules are the primary site of UGT and SULT activity in the rabbit kidney.
    • Enzyme localization suggests a significant role in proximal tubule xenobiotic metabolism.
    • Further research into UGT and SULT function in renal drug processing is warranted.

    Related Experiment Videos