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Related Experiment Video

Updated: Jan 28, 2026

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

Published on: July 9, 2008

Bone Marrow-Derived Dendritic Cells.

¹Rho, Chapel Hill, NC, USA. kelly_roney@rhoworld.com.

Methods in Molecular Biology (Clifton, N.J.)

|February 25, 2019

Summary

This summary is machine-generated.

This study presents an optimized protocol for isolating and culturing large numbers of dendritic cells from mouse bone marrow. This method aims to improve the study of these crucial antigen-presenting cells for immune system research.

Keywords:

Bone marrow Dendritic cell GM-CSF IL-4 Mouse

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Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

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Last Updated: Jan 28, 2026

Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

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Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

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Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

Published on: October 30, 2018

Area of Science:

Immunology
Cell Biology

Background:

Dendritic cells (DCs) are key antigen-presenting cells bridging innate and adaptive immunity.
Understanding DC function is vital for advancing immune system research.
Current isolation methods yield low cell numbers, hindering comprehensive study.

Purpose of the Study:

To develop an optimized protocol for isolating and culturing large quantities of dendritic cells from mouse bone marrow.
To facilitate downstream experimental applications requiring substantial numbers of dendritic cells.

Main Methods:

Bone marrow isolation from mice.
Optimized cell culture techniques for dendritic cell expansion.
Protocols designed for high-yield dendritic cell recovery.

Main Results:

Successful isolation and culture of significantly larger numbers of dendritic cells compared to standard methods.
Demonstrated viability and functionality of cultured dendritic cells for experimental use.

Conclusions:

The presented protocol effectively optimizes dendritic cell isolation and culture from mouse bone marrow.
This method provides a reliable source of numerous dendritic cells, supporting diverse immunological research.