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Decision Making01:20

Decision Making

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Decision-making is a fundamental cognitive process that involves evaluating alternatives and selecting among them. This process can range from simple choices, such as deciding what to wear, to complex decisions, like choosing a major in college or a career path. The complexity of the decision often dictates the approach we use, which can be broadly categorized into two types: automatic and controlled decision-making.
Automatic decision-making is fast, intuitive, and relies on gut feelings...
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Quantifying Work02:30

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As a system undergoes a change, its internal energy can change, and energy can be transferred from the system to the surroundings, or from the surroundings to the system.
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Decision Making: P-value Method01:09

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The process of hypothesis testing based on the P-value method includes calculating the P- value using the sample data and interpreting it.
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Decision Making: Traditional Method01:14

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The process of hypothesis testing based on the traditional method includes calculating the critical value, testing the value of the test statistic using the sample data, and interpreting these values.
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Quantifying Heat02:46

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Thermal Energy Microscopically, thermal energy is the kinetic energy associated with the random motion of atoms and molecules. Temperature is a quantitative measure of “hot” or “cold”, which depends on the amount of thermal energy. When the atoms and molecules in an object are moving or vibrating quickly, they have a higher average kinetic energy (KE) (or higher thermal energy), and the object is perceived as “hot”, or it is described as being at a higher temperature. When the...
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Quantifying and Rejecting Outliers: The Grubbs Test01:02

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Sometimes, a data set can have a recorded numerical observation that greatly  deviates from the rest of the data. Assuming that the data is normally distributed, a statistical method called the Grubbs test can be used to determine whether the observation is truly an outlier.  To perform a two-tailed Grubbs test, first, calculate the absolute difference between the outlier and the mean. Then, calculate the ratio between this difference and the standard deviation of the sample. This...
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Related Experiment Video

Updated: Jan 28, 2026

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
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Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

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Microfluidic Platform to Quantify Neutrophil Migratory Decision-Making.

Brittany P Boribong1, Amina Rahimi2, Caroline N Jones3

  • 1Genetics, Bioinformatics, and Computational Biology, Virginia Tech, Blacksburg, VA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 25, 2019
PubMed
Summary

This study introduces a new microfluidic device to precisely measure how neutrophils migrate towards infection sites. This rapid, single-cell assay offers a better way to study neutrophil chemotaxis compared to older methods.

Keywords:
ChemotaxisImmune cellMicrofluidicsMigratory decision-makingNeutrophil migration phenotype

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Author Spotlight: Quantifying Neutrophil Extracellular Traps in Disease and Drug Screening Using Dual-Color Live-Cell Imaging
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Area of Science:

  • Immunology
  • Cell Biology
  • Biomedical Engineering

Background:

  • Neutrophils are critical immune cells, acting as the first responders to infection and tissue damage.
  • Neutrophil migration, or chemotaxis, is essential for host defense but current measurement methods are cumbersome.
  • Existing assays often require large blood volumes and provide only bulk, endpoint data, limiting detailed analysis.

Purpose of the Study:

  • To develop a novel, high-resolution microfluidic platform for quantifying neutrophil chemotaxis.
  • To enable precise measurement of single neutrophil migratory phenotypes, including velocity and directionality.
  • To provide a rapid and robust alternative to traditional bulk assays for studying neutrophil behavior.

Main Methods:

  • Engineered a dual gradient microfluidic device to create precise chemokine gradients.
  • Developed a protocol for high-temporal-resolution tracking of individual neutrophils.
  • Utilized single-cell resolution to analyze neutrophil migratory decision-making.

Main Results:

  • The microfluidic platform precisely quantifies neutrophil migration dynamics.
  • Single-cell analysis reveals detailed migratory phenotypes (velocity, directionality).
  • The assay provides high temporal resolution, capturing rapid cellular responses.

Conclusions:

  • The novel microfluidic platform offers a significant advancement in studying neutrophil chemotaxis.
  • This high-resolution assay enables a deeper understanding of neutrophil migratory behavior in response to stimuli.
  • The developed protocol provides a rapid, robust, and precise tool for immunological research.