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Related Experiment Video

Updated: Jan 28, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

Methods for Natural Transformation in Acinetobacter baumannii.

Gottfried Wilharm¹, Evelyn Skiebe²

¹Robert Koch Institute, Wernigerode, Germany. wilharmg@rki.de.

Methods in Molecular Biology (Clifton, N.J.)

|February 25, 2019

Summary

Acinetobacter baumannii uses natural transformation for horizontal gene transfer (HGT), promoting multidrug resistance. This study details methods for investigating DNA uptake during natural transformation in this pathogen.

Keywords:

Acinetobacter baumannii Antibiotic resistance DNA uptake Motility Natural transformation competence Nosocomial pathogen

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The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides

Published on: November 2, 2021

Area of Science:

Microbiology
Genetics
Infectious Diseases

Background:

Acinetobacter baumannii is a significant nosocomial pathogen.
Horizontal gene transfer (HGT) drives the evolution of multidrug resistance in A. baumannii.
Natural transformation is a key mechanism for HGT.

Purpose of the Study:

To describe methods for studying DNA uptake in A. baumannii.
To elucidate the role of natural transformation in A. baumannii evolution.

Main Methods:

Detailed protocols for inducing and measuring natural transformation in A. baumannii.
Techniques for assessing DNA uptake efficiency.

Main Results:

Established methodologies for studying natural transformation competence.
Provided insights into the process of DNA acquisition via natural transformation.

Conclusions:

Understanding DNA uptake mechanisms is crucial for combating A. baumannii.
These methods facilitate further research into HGT and resistance development.