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Updated: Jan 28, 2026

Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars
Published on: August 20, 2014
BioFlux™ 200 Microfluidic System to Study A. baumannii Biofilm Formation in a Dynamic Mode of Growth
Bertrand Naudin1,2, Anja Heins3, Stéphane Pinhal3
1Normandie University, Unirouen, Rouen, France.
Abstract:
The ability of A. baumannii to develop biofilms on a wide range of surfaces can be associated to its persistence in hospital settings and the emergence of recalcitrant and chronic infections. Few compounds are available to eradicate A. baumannii biofilms, and most of them have been tested for their antibiofilm properties in static conditions. Microfluidics systems as BioFlux™ system are now available for studying A. baumannii biofilm formation in dynamic conditions. Here, we described the use of this system for studying the biofilm development of the reference strain A. baumannii ATCC 17978 in a dynamic mode. We showed how to test the activity of an antibiotic (colistin at the MIC concentration, 0.5 μg/mL) in these conditions of growth.
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