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Updated: Jan 28, 2026

Analysis of Oxidative Stress in Zebrafish Embryos
Published on: July 7, 2014
Identification of proteins in Sporothrixschenckiisensu stricto in response to oxidative stress induced by hydrogen
Estela Ruiz-Baca1, Hilda Leyva-Sánchez1, Bertha Calderón-Barraza1
1Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango, Av. Veterinaria S/N, 34120 Durango, DGO, Mexico.
Background:
Sporotrichosis is a fungal infection caused by the Sporothrix schenckii complex. In order to colonize the host, the pathogen must neutralize the reactive oxygen species produced by the phagocytic cells during the respiratory burst. Little is known about these mechanisms in S. schenckii.
Aims:
To identify the proteins differentially expressed after the exposure of S. schenckiisensu stricto to different concentrations of H2O2.
Methods:
Yeast cells of S. schenckiisensu stricto were exposed to increasing concentrations of H2O2. Proteins differentially expressed in response to oxidative stress were analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and identified by MALDI-MS/MS. RT-PCR assays were performed to evaluate the transcription of genes of the identified proteins.
Results:
Concentrations of H2O2 as high as 800mM allowed cell growth, and 200mM and 400mM were selected for comparative analysis by 2D-PAGE. This analysis revealed at least five differentially expressed proteins, which were identified as heat shock 70kDa protein (Hsp70), chaperonin GroEL, elongation factor 1-β (EF1-β), a hypothetical protein, and mitochondrial peroxiredoxin (Prx1). RT-PCR revealed that the transcription of the genes coding for some of these proteins are differentially regulated.
Conclusions:
Based on these results, it is proposed that these proteins may be involved in the resistance of S. schenckii to oxidative stress, and play an important role in the fungus survival in the host.
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