[Release of beta-alanine from the frog skeletal muscle determined by thin-layer chromatography]

The release of beta-alanine from the resting and contracting frog sartorius muscles was demonstrated by the two-dimensional thin-layer chromatography. The release of beta-alanine from indirectly stimulated muscles of frogs in winter was about 230% higher than at rest. When synaptic transmission was blocked by d-tubocurarine the release of beta-alanine from directly stimulated muscles did not exceed the release at rest. Thus, activation of neuromuscular synapse leads to increased beta-alanine release from contracting muscle.