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Updated: Jan 28, 2026

Multimodal Optical Imaging Platform for Studying Cellular Metabolism
Published on: June 6, 2025
Multimodal imaging platform for optical virtual skin biopsy enabled by a fiber-based two-color ultrafast laser source
Hsiang-Yu Chung1,2,3, Rüdiger Greinert4, Franz X Kärtner1,2,5
1Center for Free-Electron Laser Science, DESY, Notkestraße 85, 22607 Hamburg, Germany.
Abstract:
We demonstrate multimodal label-free nonlinear optical microscopy in human skin enabled by a fiber-based two-color ultrafast source. Energetic femtosecond pulses at 775 nm and 1250 nm are simultaneously generated by an Er-fiber laser source employing frequency doubling and self-phase modulation enabled spectral selection. The integrated nonlinear optical microscope driven by such a two-color femtosecond source enables the excitation of endogenous two-photon excitation fluorescence, second-harmonic generation, and third-harmonic generation in human skin. Such a 3-channel imaging platform constitutes a powerful tool for clinical application and optical virtual skin biopsy.
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