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Updated: Jan 28, 2026

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
Published on: June 2, 2009
Comparison of time- and angular-domain scatter rejection in mesoscopic optical projection tomography: a simulation
L Sinha1, F Massanes2, V C Torres1
1Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn Street, Chicago, IL 60616, USA.
None:
Optical imaging offers exquisite sensitivity and resolution for assessing biological tissue in microscopy applications; however, for samples that are greater than a few hundred microns in thickness (such as whole tissue biopsies), spatial resolution is substantially limited by the effects of light scattering. To improve resolution, time- and angular-domain methods have been developed to reject detection of highly scattered light. This work utilizes a modified version of a commonly used Monte Carlo light propagation software package (MCML) to present the first comparison of time- and angular-domain improvements in spatial resolution with respect to varying sample thickness and optical properties (absorption and scattering). Specific comparisons were made at various tissue thicknesses (1-6 mm) assuming either typical (average) soft tissue scattering properties, μs ' = 10 cm-1, or low scattering properties, μs ' = 3.4 cm-1, as measured in lymph nodes.
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