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Engineering of phosphoenolpyruvate: carbohydrate phosphotransferase system increased acetate assimilation in
Pengfei Gu1, Hao Niu1, Xiangyu Fan1
1School of Biological Science and Technology, University of Jinan, Jinan, 250022 People's Republic of China.
Abstract:
Acetate is a potential low-cost carbon source and can be used for microbial production of valuable chemicals in Escherichia coli. In this study, separate and simultaneous inactivation of the ptsG, ptsI, and ptsP genes involved in the phosphoenolpyruvate:carbohydrate phosphotransferase system was performed in E. coli and the effects on cell growth and acetate assimilation were evaluated. The mutant strain with double deletion of ptsG and ptsP exhibited faster acetate use than the other mutants. Inactivation of ptsI seriously reduced acetate consumption. This work provides a novel engineering target for improving acetate use rates.
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