Molecular cloning and expression analysis of GT-2-like genes in strawberry

GT-2 factors are the members of trihelix transcription factors (TFs) which can function in regulating plant development and responding to different abiotic stress. These proteins contain two structural domains composed by three tandem repeats helix-loop-helix-loop-helix. The strawberry (Fragaria × ananassa Duch.) is one of the most prevalent fruit crops due to its high economic and nutritional value. Nevertheless, strawberry production is limited by a range of biotic and abiotic stresses (such as drought, extreme temperature) that cause significant losses every year. Despite the potential roles of GT-2 transcription factor in plants, the functional and systematic analysis of the strawberry GT-2 subfamily has not been reported yet. In this research, we identified six GT-2 factors in 'Benihoppe' strawberry (Fragaria × ananassa) and all the FaGT-2-like proteins contain two trihelix domains. In addition, bioinformatics analysis showed that FaGT-2-like proteins might participate in transcription or transcription regulation. Compared with other reported GT-2 proteins, the similarity between FaGT-2-like and FvGT-2-like amino acid sequences was the highest, which can reach to 100%. Expression of these TFs indicated all of the FaGT-2-like genes could express in different tissues: root, stem, and leaf within distinct expression patterns. Furthermore, quantitative real-time PCR (qRT-PCR) analysis provided us with cues that all the FaGT-2-like genes were downregulated in response to various abiotic stress and hormone treatment. All the gene expressions can be inhibited by salt, drought, cold and ABA treatments, indicating that all the FaGT-2-like genes in 'Benihoppe' strawberry might act as the negative regulatory factors to respond to the abiotic stress. In summary, these results would lay a useful foundation for FaGT-2-like genes on functional study.