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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Interaction Between Sympk and Oct4 Promotes Mouse Embryonic Stem Cell Proliferation.

Jianping Yu1, Weisi Lu2, Tianyu Ge1

  • 1MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, SunYat-sen University, Guangzhou, People's Republic of China.

Stem Cells (Dayton, Ohio)
|February 26, 2019
PubMed
Summary

Symplekin (Sympk) promotes embryonic stem cell (ESC) self-renewal and pluripotency by interacting with Oct4. This interaction maintains genomic stability, suggesting Sympk

Keywords:
Embryonic stem cellsGenomic stabilityOct4PluripotencyProliferationSymplekin

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Area of Science:

  • Stem cell biology
  • Molecular and cell biology

Background:

  • Symplekin (Sympk) is a scaffold protein with known roles in RNA processing and epithelial cell regulation.
  • Its function in embryonic stem cells (ESCs), where it is highly expressed, is currently unknown.

Purpose of the Study:

  • To investigate the role of Symplekin (Sympk) in mouse embryonic stem cells (ESCs).
  • To determine Sympk's contribution to ESC self-renewal, pluripotency, and genomic stability.

Main Methods:

  • CRISPR/Cas9 gene editing to delete Sympk in mouse ESCs.
  • Assessing colony formation, teratoma formation, and chimeric mouse development to evaluate pluripotency.
  • Analyzing gene expression related to proliferation and differentiation.
  • Investigating the interaction between Sympk and Oct4 using the DUF3453 domain.

Main Results:

  • Sympk overexpression enhanced ESC colony formation and pluripotency, while Sympk deletion reduced these.
  • Sympk sustained ESC pluripotency and genomic stability during long-term culture.
  • The DUF3453 domain of Sympk is crucial for Oct4 interaction and colony formation.
  • Sympk modulated gene expression, activating proliferation genes and suppressing differentiation genes.

Conclusions:

  • Symplekin (Sympk) interacts with Oct4 to promote ESC self-renewal and pluripotency.
  • Sympk is essential for maintaining genomic integrity in ESCs.
  • Sympk holds potential for applications in stem cell therapies.