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Updated: Jan 28, 2026

Thin-layer Chromatographic TLC Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
Published on: March 27, 2014
Compounds from the root of Pueraria peduncularis (Grah. ex Benth.) Benth. and their antimicrobial effects
Huabao Chen1, Xiaomin Zhao1, Tianxing Lv1
1Department of Plant Protection, College of Agronomy, Sichuan Agricultural University, Chengdu, China.
Background:
Pueraria peduncularis belongs to the genus Pueraria DC, and has a wide range of medical and agricultural activities. Previous studies have shown that P. peduncularis extracts have broad bioactivities against phytopathogens. In this paper, we systematically studied the fungicidal activity of root methanol extracts and further isolated the active compounds.
Results:
The root methanol extract inhibited the mycelial growth of the five tested phytopathogens to different degrees. Among these phytopathogens, the inhibitory effect was greatest against R. solani, with an EC50 value of 324.72 mg L-1 . Eight compounds were subsequently isolated and identified from P. peduncularis. Among them, puercarpan A and medicarpin showed strong fungicidal activity, with MIC values against Rhizoctonia solani of 1.6 and 6.25 mg L-1 , respectively. Puercarpan A is a new compound, and its structure was established as (6aR,6bS,11aR)-6b-hydroxy-3-methoxypterocarpan-10-ene-7-one.
Conclusion:
The P. peduncularis extracts exhibit high antimicrobial activity against R. solani and have great potential value of P. peduncularis as a fungicide. © 2019 Society of Chemical Industry.
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