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PMab-213: A Monoclonal Antibody for Immunohistochemical Analysis Against Pig Podoplanin

Yukinari Kato1,2, Shinji Yamada2, Yoshikazu Furusawa1,2,3

  • 11 New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan.

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
|February 26, 2019
PubMed

Podoplanin (PDPN) is known to be expressed in normal tissues, including lymphatic endothelial cells, renal podocytes, and type I lung alveolar cells. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine PDPN have already been established; however, mAbs against pig PDPN (pPDPN) are lacking. In the present study, mice were immunized with pPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/pPDPN), and hybridomas producing mAbs against pPDPN were identified by flow cytometric screening. One of the mAbs, PMab-213 (IgG2b, kappa), could specifically detect CHO/pPDPN cells through flow cytometry and detect pPDPN through western blot analysis. KD of PMab-213 for CHO/pPDPN was determined to be 2.1 × 10-9 M, indicating a high affinity for CHO/pPDPN. Furthermore, PMab-213 strongly stained lymphatic endothelial cells, renal podocytes, and type I lung alveolar cells through immunohistochemistry. PMab-213 is expected to be useful in investigating the function of pPDPN.

Keywords:
PDPNPMab-213pig podoplanin

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