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Modified DCs and MSCs with HPV E7 antigen and small Hsps: Which one is the most potent strategy for eradication of

Azam Bolhassani1, Sepideh Shahbazi1, Elnaz Agi2

  • 1Department of Hepatitis and AIDs, Pasteur Institute of Iran, Tehran, Iran.

Molecular Immunology
|February 26, 2019
PubMed

Insights

Dendritic cells (DCs) and mesenchymal stem cells (MSCs) modified with small heat shock proteins (sHsps) and E7 oncoprotein show promise for cancer immunotherapy. These modified cells effectively stimulate immune responses and provide significant protection against tumors in mouse models.

Area of Science:

  • Immunology
  • Oncology
  • Biotechnology

Background:

  • Dendritic cells (DCs) and mesenchymal stem cells (MSCs) are explored for cancer immunotherapy.
  • Small heat shock proteins (sHsps) and oncoproteins are investigated as vaccine components.

Purpose of the Study:

  • To evaluate immune responses and anti-tumor effects of DCs and MSCs modified with sHsp27, sHsp20, and E7 oncoprotein.
  • To compare homologous and heterologous prime/boost vaccination strategies.

Main Methods:

  • DCs and MSCs were derived from mouse bone marrow.
  • Cells were modified with sHsp27, sHsp20, and E7 oncoprotein.
  • Homologous and heterologous prime/boost vaccination strategies were employed in a tumor mouse model.

Main Results:

  • DCs pulsed with E7+Hsp27/Hsp20 induced Th1 responses (IgG2a, IgG2b, IFN-γ, IL-10) and complete tumor protection.
  • MSCs transfected with E7+Hsp27 enhanced E7-specific T-cell responses and suppressed tumor growth.
  • MSCs transfected with E7+Hsp20 did not achieve complete protection.

Conclusions:

  • DC- and MSC-based vaccinations with specific modalities are effective for immunotherapy against HPV-associated cancers.
  • The choice of cell type and modification strategy impacts therapeutic efficacy.
Keywords:
Dendritic cellE7 oncoproteinHuman papillomavirusMesenchymal stem cellSmall heat shock protein

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