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Updated: Jan 28, 2026

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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DNA stretching induces Cas9 off-target activity.

Matthew D Newton1,2, Benjamin J Taylor3, Rosalie P C Driessen4

  • 1Molecular Virology, Department of Medicine, Imperial College London, London, UK.

Nature Structural & Molecular Biology
|February 27, 2019
PubMed
Summary

CRISPR/Cas9 genome editing shows specific DNA binding at low forces. Mechanical stress at higher forces reveals off-target binding, influenced by DNA structure and guide RNA, impacting therapeutic safety.

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