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Updated: Jan 28, 2026
Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
Monoclonal Antibody Production Against Vimentin by Whole Cell Immunization in a Mouse Model
Marzieh Rezaei1, Abbas Ghaderi2
1Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Background:
Pancreatic carcinoma is the fourth-leading cause of cancer death in the United States and due to its late presentation, only few patients would be candidates for the curative treatment of pancreactomy. Monoclonal antibodies have brought hope to targeted therapy.
Objectives:
To identify new biomarkers, a panel of monoclonal antibodies was generated against newly established cell line, Faraz-ICR from a patient with pancreatic acinar cell carcinoma.
Material And Methods:
Balb/c female mice were immunized with Faraz-ICR cell line and their spleenocytes fused with SP2/0 myeloma cell line. Highly reactive hybridoma producing antibodies against Faraz-ICR was detected using ELISA, immunofluorescence staining and flow cytometry. Western blot and 2D immunoblot were utilized for further characterization of the target antibodies.
Results:
Among highly reactive clones, the reactivity of 7C11 clone was assessed in comparison to other epithelial tumors. The antibody isotype was IgM that reacted with a 55 kDa protein in western blot analysis. To further characterize the target antigen, immunoproteome of the Faraz-ICR cell line was performed. By LC-MS analysis, the target of 7C11 clone was identified to be vimentin.
Conclusions:
Pancreatic cancer is a highly lethal malignancy with no reliable biomarker for early detection and diagnosis. In this study, by establishing a pancreatic acinar carcinoma cell line, a panel of monoclonal antibodies was generated to identify specific or associated cancer targets. Furthermore, 7C11 mAb was introduced that can specifically recognizes vimentin as a tumor marker. This antibody may serve as a new tool for prognostic and therapeutic strategies.
Insights
Researchers developed a new monoclonal antibody, 7C11, targeting vimentin. This antibody shows potential as a novel biomarker for pancreatic cancer, aiding in early detection and therapeutic strategies.
Area of Science:
- Oncology
- Immunology
- Biochemistry
Background:
- Pancreatic carcinoma is a leading cause of cancer death, often diagnosed late, limiting curative treatment options like pancreactomy.
- Targeted therapy using monoclonal antibodies offers a promising avenue for pancreatic cancer treatment.
Purpose of the Study:
- To generate a panel of monoclonal antibodies against a newly established pancreatic acinar cell carcinoma cell line (Faraz-ICR).
- To identify novel biomarkers for pancreatic cancer detection and diagnosis.
Main Methods:
- Immunization of mice with the Faraz-ICR cell line and generation of hybridomas.
- Screening of hybridomas using ELISA, immunofluorescence, and flow cytometry.
- Characterization of antibody targets via Western blot, 2D immunoblot, and LC-MS analysis.
Main Results:
- A specific monoclonal antibody, clone 7C11 (IgM isotype), was identified.
- Western blot analysis revealed 7C11 reacts with a 55 kDa protein.
- LC-MS analysis identified the target antigen recognized by 7C11 as vimentin.
Conclusions:
- Vimentin has been identified as a potential tumor marker for pancreatic cancer using the 7C11 monoclonal antibody.
- The 7C11 antibody may serve as a valuable tool for improving prognostic and therapeutic strategies in pancreatic cancer.
- This research contributes to the development of novel biomarkers for this lethal malignancy.
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