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Determining the Biofilm Forming Gene Profile of Staphylococcus aureus Clinical Isolates via Multiplex Colony PCR

Mostafa Shahmoradi1, Peyman Faridifar1, Reza Shapouri1

  • 1Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran.

Reports of Biochemistry & Molecular Biology
|February 27, 2019
PubMed
Abstract

Insights

This study found that most Staphylococcus aureus (S. aureus) strains from hospitalized patients form biofilms and are resistant to common antibiotics like penicillin. Identifying these antibiotic resistance genes is crucial for effective treatment.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Hospital-acquired Staphylococcus aureus (S. aureus) infections present a significant clinical challenge.
  • Understanding the genetic basis of biofilm formation and antibiotic resistance in S. aureus is critical for effective treatment strategies.

Purpose of the Study:

  • To determine the prevalence of biofilm-forming genes in clinical S. aureus isolates.
  • To assess the antibiotic susceptibility patterns of these S. aureus strains.

Main Methods:

  • Clinical samples were analyzed using biochemical assays and confirmed with multiplex PCR for S. aureus identification.
  • Biofilm formation capacity was evaluated in vitro.
  • Presence of specific genes (cna, fnbA, fnbB, pvl) and antibiotic susceptibility were tested.

Main Results:

  • Multiplex PCR identified 50 S. aureus strains; 64% were weak, 30% moderate, and 6% strong biofilm formers.
  • Prevalence of biofilm genes: cna (62%), fnbA (70%), fnbB (26%), pvl (2%).
  • All isolates were resistant to penicillin, amoxicillin, and clavulanic acid.

Conclusions:

  • The high prevalence of biofilm-producing S. aureus strains with significant antibiotic resistance underscores the need for rapid diagnostic methods.
  • Effective elimination of S. aureus infections requires timely identification of antibiotic resistance profiles, especially in biofilm-forming strains.
Keywords:
BiofilmMultiplex colony PCRPertussis toxinSpreading factorsStaphylococcus aureus

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