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[Advances in antibody drug expression techniques].

Mengxiao Zhang1, Jianwei Zhu1, Huili Lu1

  • 1Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|February 27, 2019
PubMed
Summary

Monoclonal antibody drugs are leading biopharmaceuticals for cancer and autoimmune diseases. Advances in expression technologies, including mammalian cells and cell-free systems, are driving antibody drug development.

Keywords:
CRISPRantibody drugsexpression systemmammalian cellsmanufacturing

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Area of Science:

  • Biotechnology
  • Biopharmaceutical Development
  • Drug Discovery

Background:

  • Monoclonal antibodies are a leading class of biopharmaceutical drugs.
  • They are used for treating malignant tumors and autoimmune disorders.
  • Technological advancements have significantly improved antibody drug development.

Purpose of the Study:

  • To summarize critical progress in antibody drug expression technologies.
  • To highlight recent advances in antibody drug development.
  • To provide an overview of various expression systems.

Main Methods:

  • Review of mammalian cell expression systems.
  • Analysis of Escherichia coli expression systems.
  • Examination of transgenic animal reactors.
  • Evaluation of cell-free protein synthesis systems.

Main Results:

  • Significant breakthroughs in recombinant cell line establishment.
  • Improvements in antibody expression and purification.
  • Enhanced quality control measures.
  • Progress in diverse expression platforms.

Conclusions:

  • Mammalian cell expression systems remain crucial.
  • Escherichia coli, transgenic animals, and cell-free systems offer alternative and complementary approaches.
  • Continuous innovation in expression technologies is vital for advancing antibody drug development.