Determination of the Critical Concentration of Rifabutin for Susceptibility Testing Using the Proportion Method with
Guangming Dai1, Suting Chen1, Lingling Dong1
1National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug Resistance Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.
Objective:
To determine the critical concentration of rifabutin (RFB) for susceptibility testing against Mycobacterium tuberculosis (Mtb) on Löwenstein-Jensen (L-J) medium using the proportion method.
Methods:
We used 47 strains were used to determine the critical concentration of RFB. The microplate antimicrobial assay (MABA) was used as a reference method. We used 160 strains to evaluate its correlation with the classification results derived from the MABA method. We performed antimicrobial susceptibility testing (AST) against RFB and rifampin (RIF) for 2748 other strains using the proportion method on L-J medium.
Results:
The determined critical concentration for RFB was 20 μg per mL. Identical classification as susceptible or resistant was observed in 93.8% and 92.5% strains for RFB and RIF, respectively, using the 2 different methods. The cross-resistance ratio between RFB and RIF was 72.7% in the 2748 Mtb strains.
Conclusions:
We determined that a critical concentration of 20 μg per mL RFB was reliable for AST of Mtb.
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