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Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
Published on: August 19, 2012
Dual-Bioorthogonal Molecular Tool: "Click-to-Release" and "Double-Click" Reactivity on Small Molecules and Material
Wilson Luo1, Johnny Luo2,3, Vladimir V Popik4
1Department of Chemistry and the Centre for Materials and Biomaterials Research , Western University , 1151 Richmond Street , London , Ontario N6A 5B7 , Canada.
Bioconjugate Chemistry
|February 27, 2019
Summary
Researchers developed a versatile molecular tool using four bioorthogonal chemistries for precise control over molecular attachment and release in materials science. This enables modular and tunable dynamic material properties under mild conditions.
Area of Science:
- Materials Science
- Biomaterials Research
- Chemical Biology
Background:
- Controlling molecular interactions in complex media is crucial for materials and biomaterials development.
- Existing ligation and click-and-release systems lack multifunctional capabilities for broad chemical applications.
Purpose of the Study:
- To design and demonstrate a multifunctional molecular tool capable of utilizing four distinct bioorthogonal chemistries interchangeably.
- To enable precise molecular control for attachment, replacement, and release in complex systems.
Main Methods:
- Utilized four bioorthogonal reactions: Staudinger-Bertozzi ligation (SBL), perfluoroaryl azide Staudinger reaction (PFAA-SR), strain-promoted alkyne-azide cycloaddition (SPAAC), and strain-promoted alkyne-nitrone cycloaddition (SPANC).
- Demonstrated "click-to-release" and "double-click" reactivity on small molecules and gold nanoparticles (AuNPs).
Main Results:
- Successfully functionalized 5 nm AuNPs with Rhodamine B and biotin using a "double-click" strategy.
- Showcased the potential of functionalized AuNPs as pretargeted delivery nanocarriers.
Conclusions:
- The developed multifunctional molecular tool offers modularity and tunable dynamic properties for advanced molecular and material systems.
- Enables precise manipulation of molecules and surfaces under mild, bioorthogonal conditions, advancing materials and biomaterials research.
