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Functional evaluation for adequacy of MDCK-lineage cells in influenza research

Hsin-Chung Tsai1,2, Caitlin W Lehman3, Chi-Chieh Lin4

  • 1Department of Surgery, Taichung Hospital, Ministry of Health and Welfare, 199 San Min Rd., Sec. 1, Taichung, Taiwan. taic61001@taic.mohw.gov.tw.

BMC Research Notes
|February 28, 2019
PubMed
Abstract

Insights

MDCK/London cells demonstrate superior influenza virus propagation and antiviral sensitivity compared to conventional MDCK cells. This enhanced cell line shows promise for influenza research and vaccine development.

Area of Science:

  • Virology
  • Cell Biology
  • Immunology

Background:

  • Influenza virus circulates globally, causing annual respiratory illness.
  • Madin-Darby Canine Kidney (MDCK) cells are standard for influenza research.
  • Conventional MDCK cells exhibit limitations in influenza strain infectivity and viral propagation.

Purpose of the Study:

  • To evaluate the functional adequacy of MDCK/London cells for influenza research.
  • To compare MDCK/London cells with conventional MDCK cells in viral propagation and antiviral sensitivity.

Main Methods:

  • Focus forming assays to assess viral infectivity.
  • Lactate dehydrogenase assay to measure cellular toxicity.
  • MTT assay to determine sensitivity to ribavirin.

Main Results:

  • MDCK/London cells efficiently propagated all tested influenza viruses, outperforming conventional MDCK cells.
  • Viral replication increased by three to four logs in MDCK/London cells, especially for H3N2.
  • MDCK/London cells exhibited greater sensitivity to the antiviral drug ribavirin.

Conclusions:

  • MDCK/London cell line offers enhanced viral propagation for influenza studies.
  • MDCK/London cells provide improved sensitivity to antiviral agents.
  • The MDCK/London cell line is a promising platform for influenza research and vaccine development.
Keywords:
Influenza virusMCDK/LondonMDCKMv1LuRibavirin

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