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Expression of the Phosphatase Ppef2 Controls Survival and Function of CD8+ Dendritic Cells

Markus Zwick1, Thomas Ulas2, Yi-Li Cho3

  • 1Faculty of Medicine, Biomedical Center (BMC), Institute for Immunology, LMU Munich, Planegg-Martinsried, Germany.

Frontiers in Immunology
|February 28, 2019
PubMed

Insights

Protein Phosphatase with EF-Hands 2 (Ppef2) is essential for maintaining immature CD8+ dendritic cell survival. Its down-regulation during maturation limits T cell responses, impacting immune homeostasis.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Apoptotic cell death in dendritic cells (DCs) is vital for immune homeostasis.
  • Dysregulation of DC death can lead to autoimmune diseases.
  • Mechanisms controlling DC apoptosis are not fully understood.

Purpose of the Study:

  • To investigate the role of Protein Phosphatase with EF-Hands 2 (Ppef2) in CD8+ dendritic cell survival and function.
  • To elucidate the impact of Ppef2 deficiency on DC apoptosis and T cell priming.

Main Methods:

  • Generation and analysis of Ppef2-deficient mice.
  • Flow cytometry to assess DC apoptosis and Bim accumulation.
  • In vivo studies to evaluate antigen presentation and T cell expansion.

Main Results:

  • Ppef2 is selectively expressed in CD8+ DCs and down-regulated upon maturation.
  • Ppef2 deficiency leads to increased CD8+ DC apoptosis due to Bim accumulation.
  • Ppef2-deficient CD8+ DCs exhibit impaired antigen presentation and reduced CD8+ T cell expansion.

Conclusions:

  • Ppef2 is crucial for the survival of immature CD8+ DCs.
  • Ppef2 down-regulation during DC maturation regulates T cell responses.
  • Ppef2 plays a significant role in immune homeostasis by controlling CD8+ DC apoptosis.
Keywords:
CD8 T cell primingDC-maturationapoptosiscross-presentationdendritic cellsimmune homeostasis

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