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Updated: Jan 28, 2026

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
Published on: December 19, 2020
A parallel permeability assay of peptides across artificial membranes and cell monolayers using a fluorogenic
Jumpei Morimoto1, Rei Amano, Takahiro Ono
1Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. jmorimoto@chembio.t.u-tokyo.ac.jp ssando@chembio.t.u-tokyo.ac.jp.
Abstract:
Here, we report a facile permeability assay to quantitatively evaluate the membrane permeability of multiple peptides in parallel. With a fluorogenic click reaction between azidocoumarin and a terminal alkyne tag introduced on a peptide, the peptide that crossed an artificial membrane or a cell monolayer was quantitatively detected. The method allows a rapid measurement of the permeability of multiple compounds on a plate reader even in the presence of a complex mixture of biological molecules.
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