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Sizing silver nanoparticles in chicken meat using direct slurry sampling graphite furnace atomic absorption
Dominic Brucker1, Kerstin Leopold2
1Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081, Ulm, Germany.
Analytical and Bioanalytical Chemistry
|February 28, 2019
Summary
Graphite furnace atomic absorption spectrometry (GFAAS) can accurately size silver nanoparticles (AgNPs) regardless of coatings or concentration. This method offers reproducible sizing, validated by reference materials, making it a reliable tool for nanoparticle analysis.
Area of Science:
- Analytical Chemistry
- Nanotechnology
- Materials Science
Background:
- Graphite furnace atomic absorption spectrometry (GFAAS) is a cost-efficient technique for nanoparticle (NP) analysis.
- New absorbance signal criteria, atomization delay (t_ad) and atomization rate (k_at), are being evaluated for NP detection and sizing.
- Real-world samples often contain NPs with various coatings and unknown concentrations, posing challenges for accurate analysis.
Purpose of the Study:
- To investigate the influence of different coating reagents and silver nanoparticle (AgNP) concentrations on GFAAS atomization behavior.
- To evaluate the reliability and reproducibility of GFAAS for sizing AgNPs in complex matrices.
- To validate the proposed GFAAS sizing method using certified reference materials.
Main Methods:
- Systematic study of nine different coating reagents and varying AgNP concentrations.
- Evaluation of atomization delay (t_ad) and atomization rate (k_at) parameters from absorbance signals.
- Direct slurry sampling GFAAS analysis of AgNPs in solid samples and reference materials.
- Comparison of GFAAS-derived sizes with certified values from NIST and transmission electron microscopy (TEM).
Main Results:
- Coating reagents and AgNP concentration did not significantly influence the observed atomization parameters (t_ad, k_at).
- GFAAS provided reproducible, size-dependent measurements that were independent of the NP coatings.
- Accurate sizing of AgNPs was achieved in two reference materials, with results closely matching certified values.
Conclusions:
- GFAAS, utilizing atomization signal characteristics, is a robust and reliable method for sizing AgNPs.
- The method's accuracy is unaffected by common NP coatings or varying concentrations.
- This validated GFAAS approach offers a cost-effective and high-throughput solution for nanoparticle characterization in diverse samples.
Keywords:
Atomization delayChicken meat reference materialGraphite furnace atomic absorption spectrometryNanoparticle analysis and sizing in real samplesNatural and engineered coating reagentsSilver nanoparticlesMore Related Videos
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