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Updated: Jan 28, 2026

In vitro Cell Migration and Invasion Assays
Published on: June 1, 2014
The influence of serum-supplemented culture media in a transwell migration assay
Siti Sarah Omar Zaki1, Livashini Kanesan1, Ming Yeh Danielle Leong1,2
1Neuroinflammation Group, Immunology Laboratory, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, 43400, Malaysia.
Abstract:
Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS-supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used.
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