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Updated: Jan 28, 2026

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue
Published on: June 21, 2018
Activity of NAD-Dependent Dehydrogenase Enzyme of Lactic Streptococci on Acetaldehyde and n-Hexanal 1
R H Schmidt1, L K Farron1, T R Bateh1
1Food Science and Human Nutrition Department, IFAS, University of Florida, Gainesville, Florida 32611.
Abstract:
Alcohol dehydrogenase (alcohol:NAD oxidoreductase, E.C. 1.1.1.1) activity was observed on acetaldehyde and n-hexanal in homogenized cell extract of Steptococcus lactis C2. Substrate inhibition was apparent at levels of n-hexanal above 4.0 mM. Increased centrifugal force from 12.000 × g for 20 min to 350,000 × g for 1 h resulted in increased specific activity in the cell-extract supernatant fluids. Aldehyde dehydrogenase (aldehyde: NAD oxidoreductose; E.C. 1.2.1.3) was not detected in any of the cell extracts. A possible involvement of the enzyme system with flavor modification in lactic-fermented oilseed milk is suggested.
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