Related Experiment Video
Updated: Jan 28, 2026
Rapid High Throughput Amylose Determination in Freeze Dried Potato Tuber Samples
Published on: October 14, 2013
Induction of Rishitin and Lubimin Synthesis in Potato Tuber Slices by Non-Specific Elicitors - Role of Gene
1Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1B 3X9.
Abstract:
The stress metabolites rishitin and lubimin accumulate at relatively low concentrations (5-20 ppm) in potato tuber slices subjected to various cell-disruptive treatments including heavy metal salts, sulfhydryl reagents, metabolic inhibitors, detergents, ultraviolet light and lysosomal enzymes. Cold-stored (4 C) tubers are more disposed to terpene accumulation than freshly harvested, 25-C stored and conditioned potatoes. Various inhibitors of DNA transcription and mRNA translation block terpene induction by non-specific elicitors when applied at sufficiently high concentration. However. various protein synthesis inhibitors were shown to be potent elicitors of terpene accumulation when applied at lower concentration. Actinomycin D (25 μg/ml) treatment of discs for 30 min elicits higher levels of rishitin than results from Phytophthora infestans interaction with potato (> 100 ppm). A mechanism for terpene induction based on derepression of "stress metabolite DNA" is proposed to explain the experimental data.
Related Concept Videos
01:58Cell Specific Gene Expression
01:58Cell Specific Gene Expression
01:23Role of Skin in Vitamin D Synthesis
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin...
02:36Transfer RNA Synthesis
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
01:15Dehydration Synthesis
Dehydration synthesis (also called a condensation reaction) is the chemical process in which two molecules covalently link together to form a new molecule, along with the release of a water molecule. Many physiologically important compounds form by dehydration synthesis reactions, such as complex carbohydrates, proteins, DNA, and RNA.
Synthesis of carbohydrates
Sugar molecules are covalently linked together by dehydration synthesis. During the reaction, the hydroxyl (-OH) group from...
01:42What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...