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Updated: Jan 28, 2026

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
Published on: October 13, 2015
Evaluation of the Minitek and API 20E Systems for Identification of Yersinia enterocolitica
L Restaino1, G S Grauman1, W A McCALL1
1Armour Food Research Center, 15101 North Scottsdale Road, Scottsdale, Arizona 85260.
Abstract:
The basic 12-disk Minitek system (14 tests) and the API 20E system were evaluated for identification of Yersinia enterocolitica strains isolated at various locations in the world. The API 20E system correctly identified all the Y. enterocolitica strains while the Minitek system identified 80% (20/25) of the strains. Four of the five cultures misidentified by Minitek were atypical strains isolated from vacuum-packaged meats. These four cultures were identified as Citrobacter freundii by Minitek due to a rhamnose-positive reaction. Minitek and API 20E are rapid and convenient systems which could prove to be beneficial for identifying Y. enterocolitica strains.
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