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First Detection of Phytoplasmas in Rhododendron in the Czech Republic
J Mertelik1, K Kloudova1, P Vanc1
1Research Institute for Landscape and Ornamental Gardening, 252 43 Pruhonice, Czech Republic.
Plant Disease
|March 1, 2019
Summary
Phytoplasma infection, specifically stolbur-type phytoplasma (16SrXII group), was identified in Rhododendron plants exhibiting leaf malformation and variegation. This marks the first global detection of this phytoplasma in Rhododendron species.
Area of Science:
- Plant Pathology
- Phytoplasma Research
- Molecular Diagnostics
Background:
- Rhododendron plants ('Cunningham's White' PS986) displayed leaf malformation and variegation.
- Micropropagation of affected plants yielded symptomatic and asymptomatic regenerants.
Purpose of the Study:
- To identify the causal agent of leaf malformation and variegation in Rhododendron.
- To characterize the phytoplasma detected in affected Rhododendron plants.
Main Methods:
- Nested polymerase chain reaction (PCR) was employed for phytoplasma detection.
- Restriction fragment length polymorphism (RFLP) analysis was used for phytoplasma classification.
Main Results:
- Phytoplasma infection was confirmed in symptomatic Rhododendron plants, including the original specimen and regenerants.
- Phytoplasmas detected belonged to the stolbur phytoplasma group (16SrXII), identified via RFLP analysis of PCR fragments.
- Similar symptoms and phytoplasma infections were observed in other Rhododendron hybrids (PS2716, PS2439).
Conclusions:
- This study reports the first global identification of stolbur-type phytoplasma in Rhododendron.
- Phytoplasma infection is associated with leaf malformation and variegation symptoms in Rhododendron.
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