Virulence and Genetic Variability Among Isolates of Mycosphaerella pinodes

Fifty-eight isolates of Mycosphaerella pinodes, collected from western Canada, New Zealand, France, Australia, the United Kingdom, and Ireland, were analyzed for pathogenic and genetic variation according to their virulence on six differential cultivars of field pea (AC Tamor, Bohatyre, Danto, Majoret, Miko, and Radley) and amplified fragment length polymorphism markers. The 56 isolates were classified into 15 pathotypes. Pathotype 1 consisted of 31 isolates that were virulent on all six pea differential cultivars. Pathotypes 14 and 15 consisted of eight isolates that were avirulent on all six differential cultivars or virulent on one of six differential cultivars. The analysis of molecular variance showed that 57.2% of the total variation was caused by differences among populations, and 42.8% was due to molecular diversity within populations. Phylogenetic analysis of molecular variation of isolates showed that most of the Canadian isolates and four Australian isolates formed two clustered groups, respectively, regardless of virulence on the six differential cultivars. Isolates from New Zealand were geographically clustered into two groups. However, the isolates from France, Ireland, and the United Kingdom were clustered with the Canadian isolates.