Related Experiment Video
Updated: Aug 18, 2026

In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver
Published on: May 4, 2016
Chloroperbenzoic acid induced DNA damage and peracid activation of alfatoxin B1
Abstract:
The activated form of aflatoxin B1 (AFB1) in vitro and in vitro is believed to be the 2,3-oxide, which cannot be isolated, presumably due to its reactivity. In addition to in vitro activation by crude metabolic enzymes, two chemical procedures are available, one involving oxidation with the mild organic oxidant chloroperbenzoic acid and another involving the synthesis of AFB1-2,3-dichloride, an electronic analog of AFB1-2,3-oxide. Here we show that chloroperbenzoic acid by itself can modify DNA, primarily at non-basepaired adenine and guanine residues, as revealed by 'replication block' analysis.
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