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Updated: Jan 28, 2026

Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
Published on: December 23, 2022
Bioinspired Hydrogels for 3D Organoid Culture
Abstract:
Organoids have stepped into the limelight as unique in vitro systems for modeling organ development, function and disease. This review provides a perspective on how chemically defined, bio-inspired hydrogels could be used for replacing ill-defined matrices derived from the native extracellular matrix (ECM) that are used for generating organoids in 3D stem cell culture. In particular, we propose the use of self-healing and light-responsive matrices that should afford control over the inherently stochastic self-organization process that currently underlies organoid morphogenesis. Such designer ECMs could accelerate the translation of organoid technology from the laboratory into various real-life applications.
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