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Analysis of the Type II-A CRISPR-Cas System in Streptococcus canis Isolated from Diseased Companion Animals and One

Haruno Yoshida1, Yasuto Fukushima1, Mieko Goto1

  • 1Laboratory of Infectious Diseases, Graduate School of Infection Control Sciences and Kitasato Institute for Life Sciences, Kitasato University.

Japanese Journal of Infectious Diseases
|March 1, 2019
PubMed

Insights

Whole-genome sequencing of Streptococcus canis strain TA4 revealed insights into M-like protein gene (scm) prevalence. Clustered regularly interspaced short palindromic repeats (CRISPR) array typing showed a genetic link between spacer genogroups and scm variations.

Area of Science:

  • Microbiology
  • Genomics
  • Bacterial Typing

Background:

  • Streptococcus canis causes infections in both animals and humans.
  • The M-like protein gene (scm) is a virulence factor in S. canis.
  • Whole-genome sequencing (WGS) provides comprehensive genetic information.

Purpose of the Study:

  • To determine the whole-genome sequence of Streptococcus canis strain TA4.
  • To evaluate the potential of type II-A clustered regularly interspaced short palindromic repeats (CRISPR) array-based typing.
  • To investigate the genetic relationship between CRISPR spacer genogroups and scm prevalence/polymorphisms.

Main Methods:

  • Whole-genome sequencing (WGS) of S. canis strain TA4.
  • CRISPRFinder and CRISPRCasFinder analysis for type II-A CRISPR loci.
  • PCR-based amplification and sequencing of CRISPR array regions.
  • Comparative analysis of homologous spacer sequences and genogroup assignment.

Main Results:

  • The type II-A CRISPR locus was identified in strain TA4 and another S. canis isolate.
  • 16 out of 20 S. canis isolates possessed a CRISPR array.
  • Five CRISPR genogroups (A-E) were identified; scm was absent in genogroup A.
  • A correlation was observed between genogroups C/E and scm allele type 1.

Conclusions:

  • CRISPR array-based typing is feasible for S. canis.
  • A genetic relationship exists between CRISPR spacer genogroups and scm prevalence/polymorphisms.
  • This study provides a foundation for understanding S. canis population genetics and virulence factor distribution.
Keywords:
CRISPR-CasJapanStreptococcus caniscompanion animalshuman patient

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