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The effect of TWIST silencing in metastatic chordoma cells

Esra Aydemir1, Ezgi Kaşikci1, Burcu Coşkunçelebi1

  • 1Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University , İstanbul , Turkey.

Turkish Journal of Biology = Turk Biyoloji Dergisi
|March 1, 2019
PubMed

Insights

This study reveals that the Twist gene plays a role in chordoma metastasis. Silencing Twist in chordoma cells reduced their migration and invasion, suggesting it as a potential therapeutic target.

Area of Science:

  • Oncology
  • Cancer Metastasis Research
  • Molecular Biology

Background:

  • Chordoma is a slow-growing, invasive bone tumor with metastatic potential in advanced stages.
  • Targeting genes involved in chordoma metastasis is crucial for developing new therapeutics.
  • Epithelial-mesenchymal transition (EMT) is a known driver of tumor metastasis.

Purpose of the Study:

  • To investigate the role of Twist, a key transcription factor in EMT, in chordoma metastasis.
  • To determine if targeting the TWIST gene can inhibit chordoma cell metastasis.

Main Methods:

  • The TWIST gene was silenced using short hairpin RNA in the MUG-Chor1 chordoma cell line.
  • Wound healing/gap closure and invasion assays were performed to assess metastatic potential.
  • Comparative analysis was conducted between Twist-silenced cells and a negative control group.

Main Results:

  • Chordoma cells with silenced TWIST expression exhibited significantly reduced migratory capacity.
  • Invasion assays demonstrated decreased invasive behavior in Twist-silenced MUG-Chor1 cells.
  • These findings suggest a direct link between Twist expression and chordoma cell metastasis.

Conclusions:

  • The transcription factor Twist appears to play a significant role in chordoma metastasis.
  • Targeting Twist may represent a novel therapeutic strategy for managing metastatic chordoma.
  • This research provides the first evidence for EMT's involvement in chordoma metastasis via the Twist pathway.
Keywords:
Cancerchordomaepithelial-mesenchymal transitiontwist

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