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Updated: Jan 28, 2026

Laser Micro-Irradiation to Study DNA Recruitment During S Phase
Published on: April 16, 2021
High-throughput Evaluation of Protein Migration and Localization after Laser Micro-Irradiation
Sebastian Oeck1,2, Nathalie M Malewicz3, Adam Krysztofiak4
1Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT, 06520, USA. sebastian.oeck@yale.edu.
Abstract:
DNA- and histone-related research frequently comprises the quantitative analysis of protein modifications, such as histone phosphorylation. Analysis of accumulation and disappearance of protein foci are used to monitor DNA damage and repair kinetics. If the protein of interest doesn't accumulate in foci, laser micro-irradiation of single nuclei provides an alternative method to monitor DNA repair proteins and histone dynamics at the DNA damage site. We have developed an automated evaluation tool for standardized, high-throughput analysis of micro-irradiated cells featuring single cell background subtraction and detection across multiple fluorescence channels, allowing for robust statistics.
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