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Updated: Jan 28, 2026

Induction and Analysis of Epithelial to Mesenchymal Transition
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Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

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PADI4‑mediated epithelial‑mesenchymal transition in lung cancer cells

Meiyan Liu1, Yang Qu2, Xue Teng3

  • 1Department of Internal Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150040, P.R. China.

Molecular Medicine Reports
|March 1, 2019
PubMed

Insights

Protein-arginine deiminase type-4 (PADI4) is overexpressed in lung cancer, driving cell invasion and migration. Targeting PADI4 may offer a new therapeutic strategy for lung cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Lung cancer involves complex genetic and phenotypic changes.
  • Protein-arginine deiminase type-4 (PADI4) is implicated in various cancers, but its role in lung cancer metastasis is unclear.

Purpose of the Study:

  • To investigate the expression of PADI4 and its associated signaling pathways in lung cancer metastasis.
  • To determine the effect of PADI4 on lung cancer cell invasion, migration, and epithelial-mesenchymal transition (EMT).

Main Methods:

  • Quantitative analysis of PADI4 expression in lung cancer cells.
  • Knockdown of PADI4 in A549 lung cancer cells.
  • Assessment of epithelial-mesenchymal transition (EMT) marker proteins.
  • Analysis of the Snail1/mothers against decapentaplegic homolog 3/4 (SMAD3/4) transcriptional complex.

Main Results:

  • PADI4 was found to be overexpressed in lung cancer cells.
  • Knockdown of PADI4 significantly reduced lung cancer cell invasion and migration.
  • PADI4 knockdown led to decreased expression of EMT markers and the Snail1/SMAD3/4 complex.

Conclusions:

  • PADI4 promotes lung cancer cell metastasis through mediating EMT.
  • PADI4-associated signaling represents a potential therapeutic target for lung cancer treatment.

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