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Updated: Jan 28, 2026
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Isolation of Soil Microorganisms Using iChip Technology
Published on: January 10, 2025
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Bacillus aciditolerans sp. nov., isolated from paddy soil
Meng-Jiao Ding1, Nian-Jie Shang2, Zhi-Xin Xiao3
1College of Biological Science and Technology, Hunan Agricultural University, Changsha 410128, PR China.
Summary
A new bacterial species, Bacillus aciditolerans sp. nov., was identified from a Chinese rice field. This Gram-positive bacterium exhibits unique genetic and chemotaxonomic properties, distinguishing it from known Bacillus species.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- The genus Bacillus encompasses diverse, Gram-positive, rod-shaped bacteria with significant environmental and industrial roles.
- Accurate bacterial classification is crucial for understanding microbial diversity and function.
Purpose of the Study:
- To characterize a novel bacterial isolate, strain YN-1T, from a rice field in China.
- To determine the taxonomic position of strain YN-1T within the genus Bacillus.
Main Methods:
- Isolation and cultivation of bacterial strain YN-1T.
- 16S rRNA gene sequencing for phylogenetic analysis.
- DNA-DNA hybridization and whole-genome analysis (ANI, GGDC).
- Analysis of cellular fatty acids and polar lipids (chemotaxonomic analysis).
- Phenotypic and biochemical characterization.
Main Results:
- Strain YN-1T is a Gram-positive, motile, rod-shaped bacterium with optimal growth at 37°C, pH 7.0, and 1.0% NaCl.
- 16S rRNA gene sequence similarity showed highest relatedness to Bacillus onubensis (98.0%).
- DNA-DNA hybridization (<47%), ANI (<95%), and GGDC (<70%) values confirmed YN-1T as a distinct taxon.
- Chemotaxonomic analysis revealed major fatty acids iso-C15:0, anteiso-C15:0, and C16:0, with a complex polar lipid profile.
- Physiological and biochemical tests differentiated YN-1T from closely related species.
Conclusions:
- Strain YN-1T represents a novel species within the genus Bacillus based on integrated phenotypic, genetic, and chemotaxonomic data.
- The proposed name for this new species is Bacillus aciditolerans sp. nov.
- The type strain for this novel species is YN-1T (=CCTCC AB 2017280T =JCM 32973T).
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