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Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
Published on: September 22, 2017
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In vivo detecting mouse persistent hyperplastic primary vitreous by Spectralis Optical Coherence Tomography
Qing Lian1, Minglei Zhao1, Tianzhong Li1
1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
Experimental Eye Research
|March 1, 2019
Summary
Spectralis Optical Coherence Tomography (OCT) effectively detects persistent hyperplastic primary vitreous (PHPV) in mice. OCT offers comparable sensitivity and precision to color photography and fluorescein angiography for identifying PHPV.
Area of Science:
- Ophthalmology
- Veterinary Medicine
- Medical Imaging
Background:
- Persistent hyperplastic primary vitreous (PHPV) is a congenital developmental anomaly of the eye.
- Accurate detection of PHPV in animal models is crucial for understanding its pathogenesis and testing therapies.
Purpose of the Study:
- To characterize the imaging features of mouse PHPV using Spectralis Optical Coherence Tomography (OCT).
- To compare the sensitivity and precision of OCT with color photography (CP) and Fundus Fluorescein Angiography (FFA) for detecting mouse PHPV.
Main Methods:
- Notch4-/- C57BL/6J mice (224 eyes) aged 3-7 months were studied.
- Eyes were imaged using CP, FFA, and Spectralis OCT.
- Hematoxylin and eosin (H&E) staining validated PHPV findings.
Main Results:
- CP and FFA identified fibrovascular tissues in 33 eyes.
- Spectralis OCT visualized lateral and oblique linear hyperreflective opacities in the vitreous cavity of 31 of these eyes.
- OCT precisely measured the position of PHPV in the posterior segment.
Conclusions:
- Spectralis OCT effectively detects mouse PHPV, revealing typical hyperreflective opacities in the vitreous.
- OCT demonstrates comparable sensitivity and precision to CP and FFA in identifying mouse PHPV.
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