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Adrenic acid non-enzymatic peroxidation products in biofluids of moderate preterm infants

Ángel Sánchez-Illana1, Vidhi Shah2, José David Piñeiro-Ramos1

  • 1Neonatal Research Unit, Health Research Institute La Fe, Avda Fernando Abril Martorell 106, 46026, Valencia, Spain.

Free Radical Biology & Medicine
|March 1, 2019
PubMed

Insights

This study presents a new method to measure oxidative stress biomarkers in preterm infants. The technique quantifies specific peroxidation products of adrenic acid (AdA) in blood plasma and urine.

Area of Science:

  • Biochemistry
  • Neonatal Medicine
  • Analytical Chemistry

Background:

  • Oxidative stress is critical in perinatal development, impacting signaling and biomolecule integrity.
  • Isoprostanoids and isofuranoids, products of polyunsaturated fatty acid (PUFA) peroxidation, are key oxidative stress biomarkers.
  • Quantifying these structurally similar compounds poses analytical challenges.

Purpose of the Study:

  • To develop a semiquantitative method for analyzing adrenic acid (AdA) peroxidation products.
  • To apply this method to assess oxidative stress in preterm infants.

Main Methods:

  • Development of an ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) method.
  • Application of the UPLC-MS/MS method to analyze blood plasma and urine samples.

Main Results:

  • A semiquantitative method for detecting dihomo-isoprostanes and dihomo-isofurans derived from AdA was successfully established.
  • The method provided insights into AdA peroxidation in preterm infants.

Conclusions:

  • The developed UPLC-MS/MS method offers a viable approach for assessing oxidative stress via AdA peroxidation products in neonatal samples.
  • This technique can aid in understanding the role of oxidative stress in preterm infant health.

Keywords:
Dihomo-isofuransDihomo-isoprostanesLipid peroxidationPreterm infantsUPLC-MS/MS

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