Au@Pt nanodendrites enhanced multimodal enzyme-linked immunosorbent assay

Area of Science:

• Nanomaterials Science
• Biomedical Engineering
• Analytical Chemistry

Background:

• Single-modal enzyme-linked immunosorbent assays (ELISA) are common, but multimodal platforms for enhanced diagnostics remain challenging.
• Multimodal imaging offers comprehensive and precise diagnostic information by integrating multiple detection techniques.
• Au@Pt nanodendrites possess unique photothermal and peroxidase-like properties suitable for multimodal sensing.

Purpose of the Study:

• To develop a novel multimodal enzyme-linked immunosorbent assay (M-ELISA) for simultaneous biomarker detection.
• To leverage the unique properties of Au@Pt nanodendrites in a single immunosensing platform.
• To demonstrate the feasibility of M-ELISA for accurate cardiac troponin I (cTnI) detection.

Main Methods:

• Fabrication of Au@Pt nanodendrites as a core sensing material.
• Development of a M-ELISA platform integrating photothermal, colorimetric, and ratiometric fluorescence immunoassays.
• Quantification of cardiac troponin I (cTnI) using the developed M-ELISA system.
• Validation of M-ELISA performance against clinical chemiluminescence immunoassay methods.

Main Results:

• The Au@Pt nanodendrite-based M-ELISA successfully quantified cTnI simultaneously via three distinct signal channels.
• The developed M-ELISA exhibited excellent photothermal and peroxidase-like activities.
• Results from M-ELISA showed good consistency with established chemiluminescence immunoassay methods for cTnI detection in real serum samples.

Conclusions:

• The novel M-ELISA platform based on Au@Pt nanodendrites offers a promising approach for simultaneous multimodal biomarker detection.
• This integrated immunoassay provides comprehensive diagnostic data, enhancing accuracy and precision.
• M-ELISA holds potential for advanced clinical diagnostics, particularly for cardiac biomarkers like cTnI.