Room temperature, microtray chromogenic assay of factor VIII:C

Commercial reagents of non-human origin were used to provide a room temperature, microtray chromogenic assay for factor VIII. The assay allows determination of factor VIII in the range 15-150% of normal plasma levels with a coefficient of variation of 3-4%. Haemophilic plasmas and factor VIII concentrates may also be assayed by varying the pre-dilution step. A good correlation was demonstrated with results obtained using an established one-stage clotting assay (r = 0.9985).