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Updated: Jan 28, 2026

Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments
Published on: February 9, 2020
Isolation and Culture of Oligodendrocytes
Marie-Theres Weil1,2,3, Giselheid Schulz-Ëberlin1, Chaitali Mukherjee4,5,6
1Max Planck Institute of Experimental Medicine, Goettingen, Germany.
Abstract:
Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons. When prepared and cultured according to these protocols, many essential aspects of the biology of oligodendrocytes, such as their proliferation, differentiation, and myelination, can be studied in culture.
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